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We analyzed the GS and GFAP expression of five cell lines and found that the expression levels and locations of GS and GFAP significantly varied among different cell lines (Supplementary information, Figure S1). These retinal stem cells do not express the markers of radial glial cells, RC233 or Pax234 (Figure 1L and 1M). BrdU incorporation for 24 h into cells at passage 5 and passage 34 demonstrated that the cells maintained high and stable proliferation abilities in long term culture (Figure 1E and 1H).Occasionally, some cells were observed that express III (early neuronal marker) and high levels of GS and GFAP (M cell markers), with long cellular processes (Figure 1J and 1K).
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