For HEK293 6E expression, cells were seeded at 0.8106cells/ml and cultured for 24 h in F17 media (Invitrogen). At a cell density of 1.6 1.8106cells/ml cells were transfected with 0.75mg/ml DNA and PEI MAX to a final concentration of 2.8g/ml. 24h after transfection, the cells were fed with HyPep 1510 (Kerry Biosciences) to a final concentration of 0.32% (v/v).
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